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PerSeptive Biosystems Inc biosystems 9050 synthesizer
Biosystems 9050 Synthesizer, supplied by PerSeptive Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biosystems+9050+synthesizer/pioneer+synthesizer/us08859854-506-2-4
Average 90 stars, based on 1 article reviews
biosystems 9050 synthesizer - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Chirality of peptide bond-forming condensation domains in nonribosomal peptide synthetases: the C5 domain of tyrocidine synthetase is a (D)C(L) catalyst.
Article Snippet: Nonribosomal peptides (NRP) such as the antibiotic tyrocidine have D-amino acids, introduced by epimerase (E) domains embedded within modules of the enzymatic assembly lines.. We predict that the peptide bond-forming condensation (C) domains immediately downstream of E domains are D-specific for the peptidyl donor and L-specific for the aminoacyl acceptor (CL).. To validate this prediction and establish that the C5 domain of tyrocidine synthetase is indeed CL, the apoT (thiolation) forms of module 4 (TycB3 AT4E) and module 5 (TycC1 C5AT5) were expressed.

Article Title: Method for modifying plant morphology, biochemistry and physiology
Article Snippet: With current technologies (e.g. PerSeptive Biosystems 9050 synthesizer, Applied Biosystems Model 431A Peptide Synthesizer), linear peptides of up to 50 residues can be manufactured.

Article Title: Method for modifying plant morphology, biochemistry and physiology
Article Snippet: With current technologies (e.g. PerSeptive Biosystems 9050 synthesizer, Applied Biosystems Model 431A Peptide Synthesizer), linear peptides of up to 50 residues can be manufactured.

Article Title: Cyclization of backbone-substituted peptides catalyzed by the thioesterase domain from the tyrocidine nonribosomal peptide synthetase.
Article Snippet: The excised C-terminal thioesterase (TE) domain from the multidomain tyrocidine nonribosomal peptide synthetase (NRPS) was recently shown to catalyze head-to-tail cyclization of a decapeptide thioester to form the cyclic decapeptide antibiotic tyrocidine A [Trauger, J. W., Kohli, R. M., Mootz, H. D., Marahiel, M. A., and Walsh, C. T. (2000) Nature 407, 215-218].. The peptide thioester substrate was a mimic of the TE domain’s natural, synthetase-bound substrate.. We report here the synthesis of modified peptide thioester substrates in which parts of the peptide backbone are altered either by the replacement of three amino acid blocks with a flexible spacer or by replacement of individual amide bonds with ester bonds.

Article Title: Characterization of SyrC, an aminoacyltransferase shuttling threonyl and chlorothreonyl residues in the syringomycin biosynthetic assembly line.
Article Snippet: Solid-Phase Synthesis of Octapeptide, COOH-L-Asp-L-Abu-LPhe-L-Arg-L-Dab-D-Dab-D-Ser-L-Ser-N-Ac, and Nonapeptide, COOH-L-Thr-L-Asp-L-Abu-L-Phe-L-Arg-L-Dab-D-Dab-D-Ser-LSer-N-Ac Peptide synthesis was performed with a PerSeptive Biosystems 9050 synthesizer (0.3 mM scale) by using diisopropylcarbodiimide (DIPCDI)/hydroxybenzotriazole (HOBt) chemistry.

Article Title: Method for improving plant tolerance to environmental stress
Article Snippet: With current technologies (e.g. PerSeptive Biosystems 9050 synthesizer, Applied Biosystems Model 431 A Peptide Synthesizer), linear peptides of up to 50 residues can be manufactured.

Article Title: Generality of peptide cyclization catalyzed by isolated thioesterase domains of nonribosomal peptide synthetases.
Article Snippet: The C-terminal thioesterase (TE) domains from nonribosomal peptide synthetases (NRPSs) catalyze the final step in the biosynthesis of diverse biologically active molecules.. In many systems, the thioesterase domain is involved in macrocyclization of a linear precursor presented as an acyl-S-enzyme intermediate.. The excised thioesterase domain from the tyrocidine NRPS has been shown to catalyze the cyclization of a peptide thioester substrate which mimics its natural acyl-S-enzyme substrate.

Synthesized:

Article Title: Transcytosis-Blocking Abs Elicited by an Oligomeric Immunogen Based on the Membrane Proximal Region of HIV-1 gp41 Target Non-Neutralizing Epitopes
Article Snippet: Binding parameters Data obtained through competition experiments were fitted by nonlinear regression using GraphPad Prism 4.0 in order to obtain Kd or IC50 (respectively). .. Synthetic peptides were synthesized on a PerSeptive Biosystems 9050 synthesizer at Arizona State University’s Protein Chemistry Laboratory. ..



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